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ca9 protein  (Bio-Rad)


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    Structured Review

    Bio-Rad ca9 protein
    Ca9 Protein, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca9 protein/product/Bio-Rad
    Average 90 stars, based on 1 article reviews
    ca9 protein - by Bioz Stars, 2026-05
    90/100 stars

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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    Gallus BioPharmaceuticals ca9 protein
    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
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    SAS institute ca9 protein
    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
    Ca9 Protein, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ca9 protein
    GA inhibits TNBC growth via targeting <t>CA9.</t> (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.
    Ca9 Protein, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca9 protein/product/Bio-Rad
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    Image Search Results


    GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: GA inhibits TNBC growth via targeting CA9. (a) Venn diagram showing the number of differentially expressed genes in the comparison between MDA-MB-231 and BT549. (b,c) TCGA and GEO databases show that CA9 is highly expressed in TNBC. (d) Correlation analysis between TNBC patient prognosis and CA9 expression. (e) IHC staining of TNBC patient-derived cancer tissue and adjacent tissue. Scale bar: 100 μm. (f) The quantitative analysis of CA9. (g) The protein levels of CA9 in cell lines were measured by Western blotting. (h) The predicted binding mode of GA with CA9. (i) GA binds to CA9 protein as shown by SPR. (j,k) CETSA analysis of CA9 combined with GA. (l,m) CA9 mRNA levels after GA 24 h treatment by qRT-PCR. (n,o) CA9 protein levels after GA 24 h treatment by Western blotting. (p) The related protein levels of siRNA-CA9 TNBC cells after GA 24 h treatment by Western blotting. Data are presented as mean ± SD. n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 when compared with control group.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Comparison, Expressing, Immunohistochemistry, Derivative Assay, Western Blot, Binding Assay, Quantitative RT-PCR, Control

    Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Garciniagifolone A, derived from Garcinia multiflora fruits, inhibits triple-negative breast cancer cells and organoids growth by targeting CA9

    doi: 10.3389/fcell.2026.1767397

    Figure Lengend Snippet: Model of the anti-tumor mechanism of GA in TNBC cells. GA could bind CA9 to induce early stage autophagy by blocking PI3K/AKT/mTOR signaling pathway. Moreover, GA suppressed the autophagic flux and increased the p62 to promote ROS production, which activated NLRP3/caspase-1/GSDMD/caspase-3 related pyroptosis and apoptosis to induce TNBC cells death.

    Article Snippet: The CA9 protein (Cusabio, CSB-EP614990HUc0) was coupled to the surface of CM5 chip biosensor (Cytiva, 29104988) in 10 mM sodium acetate (pH 4.5).

    Techniques: Blocking Assay